TY  - JOUR
A1  - Vierock, Johannes Tobias Theodor
A1  - Rodriguez-Rozada, Silvia
A1  - Dieter, Alexander
A1  - Pieper, Florian
A1  - Sims, Ruth
A1  - Tenedini, Federico
A1  - Bergs, Amelie
A1  - Bendifallah, Imane
A1  - Zhou, Fangmin
A1  - Zeitzschel, Nadja
A1  - Ahlbeck, Joachim
A1  - Augustin, Sandra
A1  - Sauter, Kathrin
A1  - Papagiakoumou, Eirini
A1  - Gottschalk, Alexander
A1  - Soba, Peter
A1  - Emiliani, Valentina
A1  - Engel, Andreas K.
A1  - Hegemann, Peter
A1  - Wiegert, Simon
T1  - BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons
T2  - Nature Communications
N2  - Optogenetic manipulation of neuronal activity through excitatory and inhibitory opsins has become an indispensable experimental strategy in neuroscience research. For many applications bidirectional control of neuronal activity allowing both excitation and inhibition of the same neurons in a single experiment is desired. This requires low spectral overlap between the excitatory and inhibitory opsin, matched photocurrent amplitudes and a fixed expression ratio. Moreover, independent activation of two distinct neuronal populations with different optogenetic actuators is still challenging due to blue-light sensitivity of all opsins. Here we report BiPOLES, an optogenetic tool for potent neuronal excitation and inhibition with light of two different wavelengths. BiPOLES enables sensitive, reliable dual-color neuronal spiking and silencing with single- or two-photon excitation, optical tuning of the membrane voltage, and independent optogenetic control of two neuronal populations using a second, blue-light sensitive opsin. The utility of BiPOLES is demonstrated in worms, flies, mice and ferrets.
KW  - Ion channels in the nervous system
KW  - Molecular neuroscience
KW  - Multiphoton microscopy
KW  - Optogenetics
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/63256
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-632564
SN  - 2041-1723
N1  - Open Access funding enabled and organized by Projekt DEAL.
N1  - This work was supported by the German Research Foundation, DFG (SPP1926, FOR2419/P6, SFB936/B8 to J.S.W., SFB936/A2 and SPP2041/EN533/15-1 to A.K.E., SPP1926 and SFB1315 to P.H., SFB807/P11 to A.C.F.B. and A.G.), the ‘Agence Nationale de la Recherche’ (CE16-2019 HOLOPTOGEN, CE16-0021 SLALLOM, ANR-10-LABX-65 LabEx LIFESENSES, and ANR-18-IAHU-01 *IHU FOReSIGHT to V.E.), the AXA research foundation and the European Research Council (ERC2016-StG-714762 to J.S.W., HOLOVIS-AdG to V.E., Stardust H2020 767092 to P.H.). Peter Hegemann is a Hertie Professor and supported by the Hertie Foundation.
VL  - 12.2021
IS  - art. 4527
SP  - 1
EP  - 20
PB  - Nature Publishing Group UK
CY  - [London]
ER  -