TY  - JOUR
A1  - Paul, Puneet
A1  - Mesihovic, Anida
A1  - Chaturvedi, Palak
A1  - Ghatak, Arindam
A1  - Weckwerth, Wolfram
A1  - Böhmer, Maik
A1  - Schleiff, Enrico
T1  - Structural and functional heat stress responses of chloroplasts of arabidopsis thaliana
T2  - Genes
N2  - Temperature elevations constitute a major threat to plant performance. In recent years, much was learned about the general molecular mode of heat stress reaction of plants. The current research focuses on the integration of the knowledge into more global networks, including the reactions of cellular compartments. For instance, chloroplast function is central for plant growth and survival, and the performance of chloroplasts is tightly linked to the general status of the cell and vice versa. We examined the changes in photosynthesis, chloroplast morphology and proteomic composition posed in Arabidopsis thaliana chloroplasts after a single or repetitive heat stress treatment over a period of two weeks. We observed that the acclimation is potent in the case of repetitive application of heat stress, while a single stress results in lasting alterations. Moreover, the physiological capacity and its adjustment are dependent on the efficiency of the protein translocation process as judged from the analysis of mutants of the two receptor units of the chloroplast translocon, TOC64, and TOC33. In response to repetitive heat stress, plants without TOC33 accumulate Hsp70 proteins and plants without TOC64 have a higher content of proteins involved in thylakoid structure determination when compared to wild-type plants.
KW  - chloroplasts
KW  - proteome
KW  - heat stress
KW  - translocon
KW  - ppi1
KW  - toc64
KW  - quantitative proteomics
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/54720
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-547209
SN  - 2073-4425
VL  - 11
IS  - 650
PB  - MDPI
CY  - Basel
ER  -