TY  - JOUR
A1  - Haack, Frederike Svenja
A1  - Poehlein, Anja
A1  - Kröger, Cathrin
A1  - Voigt, Christian A.
A1  - Piepenbring, Meike
A1  - Bode, Helge Björn
A1  - Daniel, Rolf
A1  - Schäfer, Wilhelm
A1  - Streit, Wolfgang R.
T1  - Molecular keys to the Janthinobacterium and Duganella spp. interaction with the plant pathogen Fusarium graminearum
T2  - Frontiers in microbiology
N2  - Janthinobacterium and Duganella are well-known for their antifungal effects. Surprisingly, almost nothing is known on molecular aspects involved in the close bacterium-fungus interaction. To better understand this interaction, we established the genomes of 11 Janthinobacterium and Duganella isolates in combination with phylogenetic and functional analyses of all publicly available genomes. Thereby, we identified a core and pan genome of 1058 and 23,628 genes. All strains encoded secondary metabolite gene clusters and chitinases, both possibly involved in fungal growth suppression. All but one strain carried a single gene cluster involved in the biosynthesis of alpha-hydroxyketone-like autoinducer molecules, designated JAI-1. Genome-wide RNA-seq studies employing the background of two isolates and the corresponding JAI-1 deficient strains identified a set of 45 QS-regulated genes in both isolates. Most regulated genes are characterized by a conserved sequence motif within the promoter region. Among the most strongly regulated genes were secondary metabolite and type VI secretion system gene clusters. Most intriguing, co-incubation studies of J. sp. HH102 or its corresponding JAI-1 synthase deletion mutant with the plant pathogen Fusarium graminearum provided first evidence of a QS-dependent interaction with this pathogen.
KW  - Janthinobacterium
KW  - Duganella
KW  - Fusarium graminearum
KW  - CAI-1
KW  - quorum sensing
KW  - violacein
KW  - bacterium-fungus interaction
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/43682
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-436820
SN  - 1664-302X
N1  - Copyright © 2016 Haack, Poehlein, Kröger, Voigt, Piepenbring, Bode, Daniel, Schäfer and Streit. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
VL  - 7
IS  - Art. 1668
SP  - 1
EP  - 17
PB  - Frontiers Media
CY  - Lausanne
ER  -