TY  - JOUR
A1  - Sommer, Katharina
A1  - Jakob, Heike
A1  - Kisch, Tobias
A1  - Henrich, Dirk
A1  - Marzi, Ingo
A1  - Frank, Johannes
A1  - Sander, Anna Lena
T1  - Local application reduces number of needed EPC for beneficial effects on wound healing compared to systemic treatment in mice
T2  - European journal of trauma and emergency surgery
N2  - Introduction: Stem cell transplantation is one of the most promising strategies to improve healing in chronic wounds as systemic administration of endothelial progenitor cells (EPC) enhances healing by promoting neovascularization and homing though a high amount of cells is needed. In the following study, we analysed whether local application can reduce the number of EPC needed achieving the same beneficial effect on wound healing.
Material and Methods: Wound healing after local or systemic treatment with EPC was monitored in vivo by creating standardized wounds on the dorsum of hairless mice measuring wound closure every second day. Systemic group received 2 × 106 EPC i.v. and locally treated group 2 × 105 EPC, locally injected. As control PBS injection was performed the same way. Expression of CD31, VEGF, CD90 and, SDF-1α was analysed immunohistochemically for evaluation of neovascularisation and amelioration of homing.
Results: Local (7.1 ± 0.45 SD) as well as systemic (6.1 ± 0.23 SD) EPC transplantation led to a significant acceleration of wound closure compared to controls (PBS local: 9.7 ± 0.5 SD, PBS systemic 10.9 ± 0.38 SD). Systemic application enhanced CD31 expression on day 6 after wounding and local EPC on 6 and 9 in comparison to control. VEGF expression was not significantly affected. Systemic and local EPC treatment resulted in a significantly enhanced SDF-1α and CD90 expression on all days investigated.
Conclusion: Local as well as systemic EPC treatment enhances wound healing. Moreover, beneficial effects are obtained with a tenfold decrease number of EPC when applied locally. Thus, local EPC treatment might be more convenient way to enhance wound healing as number of progenitor cells is limited.
KW  - Endothelial progenitor cells
KW  - Wound healing
KW  - Angiogenesis
KW  - Epithelialization
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/75129
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-751297
SN  - 1863-9941
N1  - Open Access funding enabled and organized by Projekt DEAL.
VL  - 48
IS  - 3
SP  - 1613
EP  - 1624
PB  - Springer Medizin
CY  - Heidelberg
ER  -