TY  - JOUR
A1  - Schamber, Tatjana
A1  - Binas, Oliver
A1  - Schlundt, Andreas
A1  - Wacker, Anna
A1  - Schwalbe, Harald
T1  - Characterization of structure and dynamics of the guanidine-II riboswitch from Escherichia coli by NMR spectroscopy and small-angle X-ray scattering (SAXS)
T2  - ChemBioChem
N2  - Riboswitches are regulatory RNA elements that undergo functionally important allosteric conformational switching upon binding of specific ligands. The here investigated guanidine-II riboswitch binds the small cation, guanidinium, and forms a kissing loop-loop interaction between its P1 and P2 hairpins. We investigated the structural changes to support previous studies regarding the binding mechanism. Using NMR spectroscopy, we confirmed the structure as observed in crystal structures and we characterized the kissing loop interaction upon addition of Mg2+ and ligand for the riboswitch aptamer from Escherichia coli. We further investigated closely related mutant constructs providing further insight into functional differences between the two (different) hairpins P1 and P2. Formation of intermolecular interactions were probed by small-angle X-ray scattering (SAXS) and NMR DOSY data. All data are consistent and show the formation of oligomeric states of the riboswitch induced by Mg2+ and ligand binding.
KW  - aptamers
KW  - guanidine riboswitch
KW  - NMR spectroscopy
KW  - RNA
KW  - structural biology
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/75227
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-752274
SN  - 1439-7633
N1  - Supported by the DFG in the Collaborative Research Center 902; Work at BMRZ is supported by the state of Hesse. A.S. is supported through DFG grant number SCHL2062/2-1 and by the Johanna Quandt Young Academy at Goethe (stipend number 2019/AS01). Open Access funding enabled and organized by Projekt DEAL.
VL  - 23
IS  - 3, art. e202100564
SP  - 1
EP  - 9
PB  - Wiley-VCH
CY  - Weinheim
ER  -