TY  - JOUR
A1  - Tohidnezhad, Mersedeh
A1  - Bayer, Andreas
A1  - Rašuo, Biljana
A1  - Hock, Jennifer Vanessa Phi
A1  - Kweider, Nisreen
A1  - Fragoulis, Athanassios
A1  - Sönmez, Tolga Taha
A1  - Jahr, Holger
A1  - Pufe, Thomas
A1  - Lippross, Sebastian
A1  - Giovarelli, Mirella
T1  - Platelet-released growth factors modulate the secretion of cytokines in synoviocytes under inflammatory joint disease
T2  - Mediators of inflammation
N2  - The etiology and pathogenesis of rheumatoid arthritis (RA) are marked by a complex interplay of various cell populations and is mediated by different signaling pathways. Traditionally, therapies have primarily focused on pain relief, reducing inflammation and the recovery of joint function. More recently, however, researchers have discussed the therapeutic efficacy of autologous platelet-rich plasma (PRP). The main objective of this work is to examine the influences of platelet-released growth factor (PRGF) on human synoviocytes under inflammatory conditions. Additionally, it is checked to which extend treatment with platelet concentrate influences the release of cytokines form synoviocytes. For this purpose, an in vitro RA model was created by stimulating the cells with the TNF-α. The release of cytokines was measured by ELISA. The cytokine gene expression was analyzed by real-time PCR. It has been observed that the stimulation concentration of 10 ng/ml TNF-α resulted in a significantly increased endogenous secretion and gene expression of IL-6 and TNF-α. The anti-inflammatory effect of PRGF could be confirmed through significant reduction of TNF-α and IL-1β. An induced inflammatory condition seems to cause PRGF to inhibit the release of proinflammatory cytokines. Further study is required to understand the exact effect mechanism of PRGF on synoviocytes.
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/48373
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-483731
SN  - 1466-1861
SN  - 0962-9351
N1  - Copyright © 2017 Mersedeh Tohidnezhad et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 17
IS  - Art. 1046438
SP  - 1
EP  - 9
PB  - Hindawi Publishing Corp.
CY  - Sylvania, Ohio
ER  -