TY  - JOUR
A1  - Blaheta, Roman A.
A1  - Weich, Eva
A1  - Marian, Dana
A1  - Bereiter-Hahn, Jürgen
A1  - Jones, Jon
A1  - Jonas, Dietger
A1  - Michaelis, Martin
A1  - Doerr, Hans Wilhelm
A1  - Cinatl, Jindrich
T1  - Human cytomegalovirus infection alters PC3 prostate carcinoma cell adhesion to endothelial cells, extracellular matrix
T2  - Neoplasia
N2  - The genome, antigens of human cytomegalovirus (HCMV) are frequently found in prostatic carcinoma. However, whether this infection is causative or is an epiphenomenon is not clear. We therefore investigated the ability of HCMV to promote metastatic processes, defined by tumor cell adhesion to the endothelium, extracellular matrix proteins. Experiments were based on the human prostate tumor cell line PC3, either infected with the HCMV strain Hi (HCMVHi) or transfected with cDNA encoding the HCMV-specific immediate early protein IEA1 (UL123) or IEA2 (UL122). HCMVHi upregulated PC3 adhesion to the endothelium, to the extracellular matrix proteins collagen, laminin, fibronectin. The process was accompanied by enhancement of β1-integrin surface expression, elevated levels of integrin-linked kinase, phosphorylation of focal adhesion kinase. IEA1 or IEA2 did not modulate PC3 adhesion or β1-integrin expression. Based on this in vitro model, we postulate a direct association between HCMV infection, prostate tumor transmigration, which is not dependent on IEA proteins. Integrin overexpression, combined with the modulation of integrin-dependent signalling, seems to be, at least in part, responsible for a more invasive PC3Hi tumor cell phenotype. Elevated levels of c-myc found in IEA1-transfected or IEA2-transfected PC3 cell populations might promote further carcinogenic processes through accelerated cell proliferation.
KW  - Adhesion
KW  - HCMV
KW  - integrins
KW  - oncomodulation
KW  - prostate carcinoma cells
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/75947
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-759478
SN  - 1476-5586
VL  - 8.2006
IS  - 10
SP  - 807
EP  - 816
PB  - Elsevier
CY  - Amsterdam
ER  -