TY  - JOUR
A1  - Löffler, Jan Gerrit
A1  - Deniz, Erhan
A1  - Feid, Carolin
A1  - Franz, Valentin G.
A1  - Bredenbeck, Jens
T1  - Versatile vibrational energy sensors for proteins
T2  - Angewandte Chemie. International Edition
N2  - Vibrational energy transfer (VET) is emerging as key mechanism for protein functions, possibly playing an important role for energy dissipation, allosteric regulation, and enzyme catalysis. A deep understanding of VET is required to elucidate its role in such processes. Ultrafast VIS-pump/IR-probe spectroscopy can detect pathways of VET in proteins. However, the requirement of having a VET donor and a VET sensor installed simultaneously limits the possible target proteins and sites; to increase their number we compare six IR labels regarding their utility as VET sensors. We compare these labels in terms of their FTIR, and VET signature in VET donor-sensor dipeptides in different solvents. Furthermore, we incorporated four of these labels in PDZ3 to assess their capabilities in more complex systems. Our results show that different IR labels can be used interchangeably, allowing for free choice of the right label depending on the system under investigation and the methods available.
KW  - Non-Canonical Amino Acids
KW  - Protein Dynamics
KW  - Protein Modifications
KW  - Time-Resolved Spectroscopy
KW  - Vibrational Energy Transfer
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/75684
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-756841
SN  - 1521-3773
N1  - We acknowledge funding by the Humboldt-Foundation (Sofja Kovalevskaja Award), as well as DFG funding (BR 3746/4-1, INST 161/722-1, GRK 1986 “CLiC”). Open Access funding enabled and organized by Projekt DEAL.
VL  - 61
IS  - 21, e202200648
SP  - 1
EP  - 8
PB  - Wiley-VCH
CY  - Weinheim
ER  -