TY  - INPR
A1  - Oosterheert, Wout
A1  - Blanc, Florian E. C.
A1  - Roy, Ankit
A1  - Belyy, Alexander
A1  - Hofnagel, Oliver
A1  - Hummer, Gerhard
A1  - Bieling, Peter
A1  - Raunser, Stefan
T1  - Molecular mechanisms of inorganic-phosphate release from the core and barbed end of actin filaments
T2  - bioRxiv
N2  - The release of inorganic phosphate (Pi) from actin filaments constitutes a key step in their regulated turnover, which is fundamental to many cellular functions. However, the molecular mechanisms underlying Pi release from both the core and barbed end of actin filaments remain unclear. Here, we combine cryo-EM with molecular dynamics simulations and in vitro reconstitution to demonstrate how actin releases Pi through a ‘molecular backdoor’. While constantly open at the barbed end, the backdoor is predominantly closed in filament-core subunits and only opens transiently through concerted backbone movements and rotameric rearrangements of residues close to the nucleotide binding pocket. This mechanism explains why Pi escapes rapidly from the filament end and yet slowly from internal actin subunits. In an actin variant associated with nemaline myopathy, the backdoor is predominantly open in filament-core subunits, resulting in greatly accelerated Pi release after polymerization and filaments with drastically shortened ADP-Pi caps. This demonstrates that the Pi release rate from F-actin is controlled by steric hindrance through the backdoor rather than by the disruption of the ionic bond between Pi and Mg2+ at the nucleotide-binding site. Our results provide the molecular basis for Pi release from actin and exemplify how a single, disease-linked point mutation distorts the nucleotide state distribution and atomic structure of the actin filament.
Y1  - 2023
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/73443
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-734436
IS  - 2023.03.25.534205
ER  -