TY  - JOUR
A1  - Lehto, Alina Barbara
A1  - Koch, Konrad
A1  - Brandes, Johanna
A1  - Viel, Christian
A1  - Fuchs, Marius
A1  - Klein, Jochen
T1  - ß-hydroxybutyrate improves mitochondrial function after transient ischemia in the mouse
T2  - Neurochemical research
N2  - ß-Hydroxybutyrate (BHB) is a ketone body formed in high amounts during lipolysis and fasting. Ketone bodies and the ketogenic diet were suggested as neuroprotective agents in neurodegenerative disease. In the present work, we induced transient ischemia in mouse brain by unilaterally occluding the middle cerebral artery for 90 min. BHB (30 mg/kg), given immediately after reperfusion, significantly improved the neurological score determined after 24 h. In isolated mitochondria from mouse brain, oxygen consumption by the complexes I, II and IV was reduced immediately after ischemia but recovered slowly over 1 week. The single acute BHB administration after reperfusion improved complex I and II activity after 24 h while no significant effects were seen at later time points. After 24 h, plasma and brain BHB concentrations were strongly increased while mitochondrial intermediates (citrate, succinate) were unchanged in brain tissue. Our data suggest that a single administration of BHB may improve mitochondrial respiration for 1–2 days but not for later time points. Endogenous BHB formation seems to complement the effects of exogenous BHB administration.
KW  - Complex I
KW  - Complex II
KW  - Glucose
KW  - Microdialysis
KW  - Oxidative phosphorylation
KW  - Stroke
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/69640
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-696401
SN  - 1573-6903
N1  - Open Access funding enabled and organized by Projekt DEAL. This work was supported by internal funds from Goethe University, Frankfurt, to JK.
VL  - 47
IS  - 11
SP  - 3241
EP  - 3249
PB  - Springer Science + Business Media B.V
CY  - Dordrecht
ER  -