TY  - JOUR
A1  - Sandmann, Gerhard
A1  - Schneider, Christian
A1  - Böger, Peter
T1  - A new non-radioactive assay of phytoene desaturase to evaluate bleaching herbicides
T2  - Zeitschrift für Naturforschung, C
N2  - A non-radioactive cell-free assay was developed to quantitatively determine inhibition of plant-type phytoene desaturase by bleaching herbicides. An active desaturase was prepared from an appropriately cloned E. coli transformant. Another E. coli transformant was used to produce the required phytoene. Phytofluene and t-carotene, the products of the desaturase reaction, were either determined by HPLC or optical absorption spectra. Enzyme kinetics and inhibition data for the bleaching tetrazole herbicide WL110547 are presented as an example.
KW  - Phytoene
KW  - Phvtofluene
KW  - ζ-Carotene
KW  - Phvtoene Desaturase
KW  - in-vitro Assay
KW  - I50 Value
KW  - WL110547
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/79942
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-799425
SN  - 0939-5075
SN  - 1865-7125
VL  - 51.1996
IS  - 7-8
SP  - 534
EP  - 538
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -