TY  - JOUR
A1  - Ritter, Andreas Hans
A1  - Safdar, Babek Khan
A1  - Jasmer, Britta
A1  - Kreis, Nina-Naomi
A1  - Friemel, Alexandra
A1  - Roth, Susanne
A1  - Solbach, Christine
A1  - Louwen, Frank
A1  - Yuan, Juping
T1  - The function of oncogene B-cell lymphoma 6 in the regulation of the migration and invasion of trophoblastic cells
T2  - International journal of molecular sciences
N2  - Human placentation is a highly invasive process. Deficiency in the invasiveness of trophoblasts is associated with a spectrum of gestational diseases, such as preeclampsia (PE). The oncogene B-cell lymphoma 6 (BCL6) is involved in the migration and invasion of various malignant cells. Intriguingly, its expression is deregulated in preeclamptic placentas. We have reported that BCL6 is required for the proliferation, survival, fusion, and syncytialization of trophoblasts. In the present work, we show that the inhibition of BCL6, either by its gene silencing or by using specific small molecule inhibitors, impairs the migration and invasion of trophoblastic cells, by reducing cell adhesion and compromising the dynamics of the actin cytoskeleton. Moreover, the suppression of BCL6 weakens the signals of the phosphorylated focal adhesion kinase, Akt/protein kinase B, and extracellular regulated kinase 1/2, accompanied by more stationary, but less migratory, cells. Interestingly, transcriptomic analyses reveal that a small interfering RNA-induced reduction of BCL6 decreases the levels of numerous genes, such as p21 activated kinase 1, myosin light chain kinase, and gamma actin related to cell adhesion, actin dynamics, and cell migration. These data suggest BCL6 as a crucial player in the migration and invasion of trophoblasts in the early stages of placental development through the regulation of various genes associated with the migratory machinery.
KW  - BCL6
KW  - trophoblast
KW  - cell and focal adhesion
KW  - actin dynamics
KW  - migration and invasion
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62360
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-623609
SN  - 1422-0067
VL  - 21
IS  - 21, art. 8393
SP  - 1
EP  - 21
PB  - Molecular Diversity Preservation International
CY  - Basel
ER  -