TY  - JOUR
A1  - Wacker, Anna
A1  - Weigand, Julia
A1  - Akabayov, Sabine R.
A1  - Altınçekiç, Nadide
A1  - Bains, Jasleen Kaur
A1  - Banijamali, Elnaz
A1  - Binas, Oliver
A1  - Castillo-Martinez, Jesus
A1  - Çetiner, Erhan Can
A1  - Ceylan, Betül
A1  - Chiu, Liang-Yuan
A1  - Davila-Calderon, Jesse
A1  - Dhamotharan, Karthikeyan
A1  - Duchardt-Ferner, Elke
A1  - Ferner, Jan
A1  - Frydman, Lucio
A1  - Fürtig, Boris
A1  - Gallego, José
A1  - Grün, J. Tassilo
A1  - Hacker, Carolin
A1  - Haddad, Christina
A1  - Hähnke, Martin Jens
A1  - Hengesbach, Martin
A1  - Hiller, Fabian
A1  - Hohmann, Katharina Felicitas
A1  - Hymon, Daniel
A1  - de Jesus, Vanessa
A1  - Jonker, Henry
A1  - Keller, Heiko
A1  - Knežić, Božana
A1  - Landgraf, Tom
A1  - Löhr, Frank
A1  - Luo, Le
A1  - Mertinkus, Klara Rebecca
A1  - Muhs, Christina
A1  - Novakovic, Mihajlo
A1  - Oxenfarth, Andreas
A1  - Palomino-Schätzlein, Martina
A1  - Petzold, Katja
A1  - Peter, Stephen
A1  - Pyper, Dennis Joshua
A1  - Qureshi, Nusrat
A1  - Riad, Magdalena
A1  - Richter, Christian
A1  - Saxena, Krishna
A1  - Schamber, Tatjana
A1  - Scherf, Tali
A1  - Schlagnitweit, Judith
A1  - Schlundt, Andreas
A1  - Schnieders, Robbin
A1  - Schwalbe, Harald
A1  - Simba-Lahuasi, Alvaro
A1  - Sreeramulu, Sridhar
A1  - Stirnal, Elke
A1  - Sudakov, Alexey
A1  - Tants, Jan-Niklas
A1  - Tolbert, Blanton S.
A1  - Vögele, Jennifer
A1  - Weiß, Lena
A1  - Wirmer-Bartoschek, Julia
A1  - Wirtz Martin, Maria Alexandra
A1  - Wöhnert, Jens
A1  - Zetzsche, Heidi
T1  - Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy
T2  - Nucleic acids research
N2  - The current pandemic situation caused by the Betacoronavirus SARS-CoV-2 (SCoV2) highlights the need for coordinated research to combat COVID-19. A particularly important aspect is the development of medication. In addition to viral proteins, structured RNA elements represent a potent alternative as drug targets. The search for drugs that target RNA requires their high-resolution structural characterization. Using nuclear magnetic resonance (NMR) spectroscopy, a worldwide consortium of NMR researchers aims to characterize potential RNA drug targets of SCoV2. Here, we report the characterization of 15 conserved RNA elements located at the 5′ end, the ribosomal frameshift segment and the 3′-untranslated region (3′-UTR) of the SCoV2 genome, their large-scale production and NMR-based secondary structure determination. The NMR data are corroborated with secondary structure probing by DMS footprinting experiments. The close agreement of NMR secondary structure determination of isolated RNA elements with DMS footprinting and NMR performed on larger RNA regions shows that the secondary structure elements fold independently. The NMR data reported here provide the basis for NMR investigations of RNA function, RNA interactions with viral and host proteins and screening campaigns to identify potential RNA binders for pharmaceutical intervention.
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/63305
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-633055
SN  - 1362-4962
N1  - Funding: Goethe University; Deutsche Forschungsgemeinschaft (DFG) [CRC902]; National Institutes of Health [U54AI50470, R01GM126833 B.S.T.]; EU Horizon 2020 [828946]; Weizmann's Internal Coronavirus Fund; German-Israel Foundation [G-1501-302]; Spanish Ministerio de Economía y Competitividad [RTI2018-093935-B-I00]; la Caixa Banking Foundation; Catholic University of Valencia; Hessisches Ministerium für Wissenschaft und Kunst [BMRZ]; iNEXT-Discovery [871037]. Funding for open access charge: DFG.
N1  - This paper is linked to: doi:10.1093/nar/gkaa1053
N1  - Chemical shifts are deposited at BMRB. All experimental data are deposited and can be downloaded at http://covid19-nmr.de.
VL  - 48
IS  - 22
SP  - 12415
EP  - 12435
PB  - Oxford Univ. Press
CY  - Oxford
ER  -