TY  - JOUR
A1  - Verboket, René
A1  - Leiblein, Maximilian
A1  - Janko, Maren Carina
A1  - Schaible, Alexander
A1  - Brune, Jan C.
A1  - Schröder, Katrin
A1  - Heilani, Myriam Widad
A1  - Fremdling, Charlotte
A1  - Busche, Yannic
A1  - Irrle, Tanja
A1  - Marzi, Ingo
A1  - Nau, Christoph Heinrich Eugen
A1  - Henrich, Dirk
T1  - From two stages to one: acceleration of the induced membrane (Masquelet) technique using human acellular dermis for the treatment of non-infectious large bone defects
T2  - European journal of trauma and emergency surgery
N2  - Introduction: The induced membrane technique for the treatment of large bone defects is a two-step procedure. In the first operation, a foreign body membrane is induced around a spacer, then, in the second step, several weeks or months later, the spacer is removed and the Membrane pocket is filled with autologous bone material. Induction of a functional biological membrane might be avoided by initially using a biological membrane. In this study, the effect of a human acellular dermis (hADM, Epiflex, DIZG gGmbH) was evaluated for the treatment of a large (5 mm), plate-stabilised femoral bone defect.
Material and Methods: In an established rat model, hADM was compared to the two-stage induced membrane technique and a bone defect without membrane cover. Syngeneous spongiosa from donor animals was used for defect filling in all groups. The group size in each case was n = 5, the induction time of the membrane was 3–4 weeks and the healing time after filling of the defect was 8 weeks.
Results: The ultimate loads were increased to levels comparable with native bone in both membrane groups (hADM: 63.2% ± 29.6% of the reference bone, p < 0.05 vs. no membrane, induced membrane: 52.1% ± 25.8% of the reference bone, p < 0.05 vs. no membrane) and were significantly higher than the control group without membrane (21.5%). The membrane groups were radiologically and histologically almost completely bridged by new bone formation, in contrast to the control Group where no closed osseous bridging could be observed.
Conclusion: The use of the human acellular dermis leads to equivalent healing results in comparison to the two-stage induced membrane technique. This could lead to a shortened therapy duration of large bone defects.
KW  - Induced membrane technique
KW  - Masquelet technique
KW  - Acellular dermis
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/69240
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-692401
SN  - 1863-9941
N1  - Open Access funding provided by Projekt DEAL.
N1  - Funding: AO-Germany Research fellowship 2017 (Maximilian Leiblein), DIZG, Berlin, Germany and German Institute for Cell and Tissue Replacement.
VL  - 46
IS  - 2
SP  - 317
EP  - 327
PB  - Springer
CY  - Berlin ; Heidelberg
ER  -