TY  - JOUR
A1  - Söhling, Nicolas
A1  - Ondreka, Muriel
A1  - Kontradowitz, Kerstin
A1  - Reichel, Tobias
A1  - Marzi, Ingo
A1  - Henrich, Dirk Markus
T1  - Early immune response in foreign body reaction is implant/material specific
T2  - Materials
N2  - The design of novel biomaterials should directly influence the host-immune system and steer it towards high biocompatibility. To date, new implants/materials have been tested for biocompatibility in vitro in cell cultures and in vivo in animal models. The current methods do not reflect reality (cell cultures) or are very time-consuming and deliver results only after weeks (animal model). In this proof-of-concept study, the suitability of a Whole Blood Stimulation Assay (WBSA) in combination with a Protein Profiler Array (PPA), as a readily available and cost-effective screening tool, was investigated. Three different biomaterials based on poly(lactic-co-glycolic acid (PLGA), calcium sulphate/-carbonate (CS) and poly(methyl methacrylate) (PMMA) were exposed to native whole blood from three volunteers and subsequently screened with a PPA. Individual reproducible protein profiles could be detected for all three materials after 24 h of incubation. The most intense reaction resulted from the use of PLGA, followed by CS. If even marginal differences in implants can be reflected in protein profiles, the combination of WBSA and PPA could serve as an early biocompatibility screening tool in the development of novel biomaterials. This may also lead to a reduction in costs and the amount of animal testing required.
KW  - PLGA
KW  - foreign body reaction
KW  - scaffold
KW  - bone tissue engineering
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/79598
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-795984
SN  - 1996-1944
N1  - The project was funded by Heraeus Medical GmbH, Wehrheim, Germany (GCM-18579).
VL  - 15
IS  - 6, art. 2195
SP  - 1
EP  - 18
PB  - MDPI
CY  - Basel
ER  -