TY  - JOUR
A1  - Lauer, Alina
A1  - Wolf, Philipp
A1  - Mehler, Dorothea
A1  - Götz, Hermann
A1  - Rüzgar, Mehmet
A1  - Baranowski, Andreas
A1  - Henrich, Dirk
A1  - Rommens, Pol Maria
A1  - Ritz, Ulrike
T1  - Biofabrication of SDF-1 functionalized 3D-printed cell-free scaffolds for bone tissue regeneration
T2  - International journal of molecular sciences
N2  - Large segmental bone defects occurring after trauma, bone tumors, infections or revision surgeries are a challenge for surgeons. The aim of our study was to develop a new biomaterial utilizing simple and cheap 3D-printing techniques. A porous polylactide (PLA) cylinder was printed and functionalized with stromal-derived factor 1 (SDF-1) or bone morphogenetic protein 7 (BMP-7) immobilized in collagen type I. Biomechanical testing proved biomechanical stability and the scaffolds were implanted into a 6 mm critical size defect in rat femur. Bone growth was observed via x-ray and after 8 weeks, bone regeneration was analyzed with µCT and histological staining methods. Development of non-unions was detected in the control group with no implant. Implantation of PLA cylinder alone resulted in a slight but not significant osteoconductive effect, which was more pronounced in the group where the PLA cylinder was loaded with collagen type I. Addition of SDF-1 resulted in an osteoinductive effect, with stronger new bone formation. BMP-7 treatment showed the most distinct effect on bone regeneration. However, histological analyses revealed that newly formed bone in the BMP-7 group displayed a holey structure. Our results confirm the osteoinductive character of this 3D-biofabricated cell-free new biomaterial and raise new options for its application in bone tissue regeneration.
KW  - bone tissue regeneration
KW  - 3D printed cell-free scaffold
KW  - polylactide
KW  - collagen type I
KW  - stromal-derived factor 1
KW  - in vivo model of critical size defects
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/54460
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-544607
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
VL  - 21
IS  - 2175
PB  - MDPI
CY  - Basel
ER  -