TY  - JOUR
A1  - Eißmann, Moritz
A1  - Gutschner, Tony
A1  - Hämmerle, Monika
A1  - Günther, Stefan
A1  - Caudron-Herge, Maïwen
A1  - Groß, Matthias
A1  - Schirmacher, Peter
A1  - Rippe, Karsten
A1  - Braun, Thomas
A1  - Diederichs, Sven
A1  - Zörnig, Martin
T1  - Loss of the abundant nuclear non-coding RNA MALAT1 is compatible with life and development
T2  - RNA biology
N2  - The metastasis-associated lung adenocarcinoma transcript 1, MALAT1, is a long non-coding RNA (lncRNA) that has been discovered as a marker for lung cancer metastasis. It is highly abundant, its expression is strongly regulated in many tumor entities including lung adenocarcinoma and hepatocellular carcinoma as well as physiological processes, and it is associated with many RNA binding proteins and highly conserved throughout evolution. The nuclear transcript MALAT-1 has been functionally associated with gene regulation and alternative splicing and its regulation has been shown to impact proliferation, apoptosis, migration and invasion.

Here, we have developed a human and a mouse knockout system to study the loss-of-function phenotypes of this important ncRNA. In human tumor cells, MALAT1 expression was abrogated using Zinc Finger Nucleases. Unexpectedly, the quantitative loss of MALAT1 did neither affect proliferation nor cell cycle progression nor nuclear architecture in human lung or liver cancer cells. Moreover, genetic loss of Malat1 in a knockout mouse model did not give rise to any obvious phenotype or histological abnormalities in Malat1-null compared with wild-type animals. Thus, loss of the abundant nuclear long ncRNA MALAT1 is compatible with cell viability and normal development.
KW  - long non-coding RNA
KW  - MALAT1
KW  - human knockout model
KW  - knockout mouse
Y1  - 2012
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/37257
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-372571
SN  - 1555-8584
SN  - 1547-6286
N1  - Copyright © 2012 Landes Bioscience. This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
Permission is granted subject to the terms of the License under which the work was published. Please check the License conditions for the work which you wish to reuse. Full and appropriate attribution must be given. This permission does not cover any third party copyrighted material which may appear in the work requested.
VL  - 9
IS  - 8
SP  - 1076
EP  - 1087
PB  - Taylor & Francis
CY  - Philadelphia, Pa.
ER  -