TY  - JOUR
A1  - Bolz, Mathias
A1  - Scheffer, Ute
A1  - Kalden, Elisabeth
A1  - Göbel, Michael
T1  - Redirection of the transcription factor SP1 to AT rich binding sites by a synthetic adaptor molecule
T2  - Helvetica chimica acta
N2  - The ubiquitous transcription factor SP1 binds to a GC rich consensus sequence. Here we describe an adaptor molecule that mediates binding of SP1 to a non-cognate DNA site rich in AT. The adaptor is comprised of a Dervan-type hairpin polyamide with high affinity to an AT rich hexamer duplex. It also carries a 27mer DNA that contains the SP1 consensus sequence. The synthesis and purification of the polyamide-DNA conjugate is reported. Pulldown experiments and western blot analysis demonstrate adaptor mediated binding of SP1 to the hexamer duplex TTGTTA.
KW  - adaptor mediated recruitment
KW  - DNA conjugate
KW  - DNA recognition
KW  - hairpin polyamides
KW  - protein capture
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62652
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-626529
SN  - 1522-2675
N1  - Open access funding enabled and organized by Projekt DEAL.
VL  - 104
IS  - 9, art. e2100095
SP  - 1
EP  - 9
PB  - Wiley-VCH
CY  - New York, NY
ER  -