TY  - JOUR
A1  - Schmidt von Braun, Serena
A1  - Sabetti, Antonino
A1  - Hanic-Joyce, Pamela J.
A1  - Gu, Jun
A1  - Schleiff, Enrico
A1  - Joyce, Paul B. M.
T1  - Dual targeting of the tRNA nucleotidyltransferase in plants: not just the signal
T2  - The journal of experimental botany : an official organ of the Society for Experimental Biology
N2  - Enzymes involved in tRNA maturation are essential for cytosolic, mitochondrial, and plastid protein synthesis and are therefore localized to these different compartments of the cell. Interestingly, only one isoform of tRNA nucleotidyltransferase (responsible for adding the 3′-terminal cytidine–cytidine–adenosine to tRNAs) has been identified in plants. The present study therefore explored how signals contained on this enzyme allow it to be distributed among the different cell compartments. It is demonstrated that the N-terminal portion of the protein acts as an organellar targeting signal and that differential use of multiple in-frame start codons alters the localization of the protein. Moreover, it is shown that the mature domain has a major impact on the distribution of the protein within the cell. These data indicate that regulation of dual localization involves not only specific N-terminal signals, but also additional factors within the protein or the cell.
KW  - Dual targeting
KW  - plant protein distribution
KW  - targeting signal
KW  - tRNA nucleotidyltransferase
Y1  - 2012
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/24781
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-247819
SN  - 1460-2431
SN  - 0022-0957
VL  - 58
IS  - 15-16
SP  - 4083
EP  - 4093
PB  - Oxford Univ. Press
CY  - Oxford
ER  -