TY  - JOUR
A1  - Streit, Deniz
A1  - Shanmugam, Thiruvenkadam
A1  - Garbelyanski, Asen
A1  - Simm, Stefan
A1  - Schleiff, Enrico
T1  - The existence and localization of nuclear snoRNAs in Arabidopsis thaliana revisited
T2  - Plants
N2  - Ribosome biogenesis is one cell function-defining process. It depends on efficient transcription of rDNAs in the nucleolus as well as on the cytosolic synthesis of ribosomal proteins. For newly transcribed rRNA modification and ribosomal protein assembly, so-called small nucleolar RNAs (snoRNAs) and ribosome biogenesis factors (RBFs) are required. For both, an inventory was established for model systems like yeast and humans. For plants, many assignments are based on predictions. Here, RNA deep sequencing after nuclei enrichment was combined with single molecule species detection by northern blot and in vivo fluorescence in situ hybridization (FISH)-based localization studies. In addition, the occurrence and abundance of selected snoRNAs in different tissues were determined. These approaches confirm the presence of most of the database-deposited snoRNAs in cell cultures, but some of them are localized in the cytosol rather than in the nucleus. Further, for the explored snoRNA examples, differences in their abundance in different tissues were observed, suggesting a tissue-specific function of some snoRNAs. Thus, based on prediction and experimental confirmation, many plant snoRNAs can be proposed, while it cannot be excluded that some of the proposed snoRNAs perform alternative functions than are involved in rRNA modification
KW  - snoRNAs
KW  - A. thaliana
KW  - cell fractionation
KW  - NGS
KW  - tissue specificity
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/55342
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-553429
SN  - 2223-7747
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 9
IS  - 8, art. 1016
SP  - 1
PB  - MDPI
CY  - Basel
ER  -