TY  - JOUR
A1  - Silva, Manassés Daniel da
A1  - Oliveira Silva, Roberta Lane de
A1  - Ferreira Neto, José Ribamar Costa
A1  - Ribeiro Guimarães, Ana Carolina
A1  - Truffi Veiga, Daniela
A1  - Moutinho Chabregas, Sabrina
A1  - Burnquist, William Lee
A1  - Kahl, Günter
A1  - Benko-Iseppon, Ana Maria
A1  - Kido, Ederson Akio
T1  - Expression analysis of sugarcane aquaporin genes under water deficit
T2  - Journal of Nucleic Acids
N2  - The present work is a pioneer study specifically addressing the aquaporin transcripts in sugarcane transcriptomes. Representatives of the four aquaporin subfamilies (PIP, TIP, SIP, and NIP), already described for higher plants, were identified. Forty-two distinct aquaporin isoforms were expressed in four HT-SuperSAGE libraries from sugarcane roots of drought-tolerant and -sensitive genotypes, respectively. At least 10 different potential aquaporin isoform targets and their respective unitags were considered to be promising for future studies and especially for the development of molecular markers for plant breeding. From those 10 isoforms, four (SoPIP2-4, SoPIP2-6, OsPIP2-4, and SsPIP1-1) showed distinct responses towards drought, with divergent expressions between the bulks from tolerant and sensitive genotypes, when they were compared under normal and stress conditions. Two targets (SsPIP1-1 and SoPIP1-3/PIP1-4) were selected for validation via RT-qPCR and their expression patterns as detected by HT-SuperSAGE were confirmed. The employed validation strategy revealed that different genotypes share the same tolerant or sensitive phenotype, respectively, but may use different routes for stress acclimation, indicating the aquaporin transcription in sugarcane to be potentially genotype-specific.
Y1  - 2013
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/32964
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-329648
SN  - 2090-021X
SN  - 2090-0201
N1  - Copyright © 2013 Manassés Daniel da Silva et al. This is an open access article distributed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/ , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 2013
IS  - Article ID 763945
SP  - 1
EP  - 14
PB  - Hindawi
CY  - New York, NY
ER  -