TY  - JOUR
A1  - Nicolas, Adele Mikheal
A1  - Pešić, Marina
A1  - Rödel, Franz
A1  - Fokas, Emmanouil
A1  - Greten, Florian
T1  - Image-guided radiotherapy in an orthotopic mouse model of rectal cancer
T2  - STAR Protocols
N2  - Radiobiology research in rectal cancer has been limited to cell lines, patient-derived organoids (PDOs), or xenografts. Here, we describe a protocol which recapitulates more efficiently the complex contributions of the tumor microenvironment. This approach establishes a preclinical mouse model of rectal cancer by intrarectal transplantation of genetically modified organoids into immunocompetent mice followed by precise image-guided radiotherapy (IGRT) of organoid-induced tumors. This model represents a useful platform to study the cellular and molecular determinants of therapy resistance in rectal cancer.
KW  - Cancer
KW  - Health sciences
KW  - Model organisms
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/78298
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-782987
SN  - 2666-1667
VL  - 3
IS  - 4, 101749
PB  - Elsevier
CY  - Amsterdam
ER  -