TY  - JOUR
A1  - Schmidtko, Achim
A1  - Gao, Wei
A1  - Sausbier, Matthias
A1  - Grundei, Inga
A1  - Niederberger, Ellen
A1  - Scholich, Klaus
A1  - Huber, Andrea
A1  - Allescher, Hans-Dieter
A1  - Hofmann, Franz
A1  - Ruth, Peter
A1  - Geisslinger, Gerd
T1  - Cysteine-rich protein 2 is a downstream effector of cGMP-dependent protein kinase I in nociception : poster presentation
T2  - BMC pharmacology
N2  - The experience of pain is mediated by a specialized sensory system, the nociceptive system. There is considerable evidence that the cGMP/cGMP kinase I (cGKI) signaling pathway modulates the nociceptive processing within the spinal cord. However, downstream targets of cGKI in this context have not been identified to date. In this study we investigated whether cysteine-rich protein 2 (CRP2) is a downstream effector of cGKI in the spinal cord and is involved in nociceptive processing. Immunohistochemistry of the mouse spinal cord revealed that CRP2 is expressed in superficial laminae of the dorsal horn. CRP2 is colocalized with cGKI and with markers of primary afferent C fibers. Importantly, the majority of CRP2 mRNA-positive dorsal root ganglion (DRG) neurons express cGKI and CRP2 is phosphorylated in a cGMP-dependent manner. To elucidate the functional role of CRP2 in nociception, we investigated the nociceptive behavior of CRP2-deficient (CRP2-/-) mice. Touch perception and acute thermal nociception were unaltered in CRP2-/- mice. However, CRP2-/- mice showed an increased nociceptive behavior in models of persistent pain as compared to wild type mice. Intrathecal administration of cGKI activating cGMP analogs increased the nociceptive behavior in wild type but not in CRP2-/- mice, indicating that the presence of CRP2 was essential for cGMP/cGKI-mediated nociception. These data indicate that CRP2 is a new downstream effector of cGKI-mediated spinal nociceptive processing and point to an inhibitory role of CRP2 in the generation of inflammatory pain.
Y1  - 2007
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/729
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-47218
N1  - © 2007 Schmidtko et al; licensee BioMed Central Ltd.
VL  - 7(Suppl 1)
IS  - P52
SP  - 1
EP  - 1
ER  -