TY  - JOUR
A1  - Sandmann, Gerhard
A1  - Hilgenberg, Wilhelm
A1  - Böger, Peter
T1  - Enzymatic preparation of 14C-labeled phytoene, squalene, and geranylgeranyl pyrophosphate from [2-14C]mevalonic ccid
T2  - Zeitschrift für Naturforschung, C
N2  - Methods are described for an enzymatic preparation of 14C-labeled terpenoids. With a cell-free system of a white mutant of Phycomyces blakesleeanus (Mucoraceae) [14C]squalene and [14C- cis]phytoene can be synthesized from [2-14C]mevalonate. The application of norflurazon, a phenyl- pyridazinone herbicide, helps to increase the yield of squalene. Furthermore, the liquid endosperm of Echinocystis lobata (Cucurbitaceae) was used for the formation of either [14C(-)]kaurene from [14C]mevalonic acid or [14C-/ra/w]geranylgeranyl pyrophosphate in the presence of Amo 1618.
The hydrocarbons formed were purified by alumina-column chromatography and preparative thin-layer chromatography (TLC). Geranylgeranyl pyrophosphate was separated by DE-column chromatography followed by TLC.
KW  - 14C-Labeled Terpenoids
KW  - cis`-Phytoene
KW  - Kaurene
KW  - Squalene
KW  - Geranylgeranyl Pyrophosphate
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/71393
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-713935
SN  - 0939-5075
SN  - 1865-7125
VL  - 35.1980
IS  - 11-12
SP  - 927
EP  - 930
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -