TY  - JOUR
A1  - Collauto, Alberto
A1  - Bülow, Sören von
A1  - Gophane, Dnyaneshwar B.
A1  - Saha, Subham
A1  - Stelzl, Lukas S.
A1  - Hummer, Gerhard
A1  - Sigurdsson, Snorri Thor
A1  - Prisner, Thomas F.
T1  - Compaction of RNA duplexes in the cell
T2  - Angewandte Chemie
N2  - The structure and flexibility of RNA depends sensitively on the microenvironment. Using pulsed electron-electron double-resonance (PELDOR)/double electron-electron resonance (DEER) spectroscopy combined with advanced labeling techniques, we show that the structure of double-stranded RNA (dsRNA) changes upon internalization into Xenopus lævis oocytes. Compared to dilute solution, the dsRNA A-helix is more compact in cells. We recapitulate this compaction in a densely crowded protein solution. Atomic-resolution molecular dynamics simulations of dsRNA semi-quantitatively capture the compaction, and identify non-specific electrostatic interactions between proteins and dsRNA as a possible driver of this effect.
KW  - EPR spectroscopy
KW  - molecular dynamics
KW  - PELDOR/DEER spectroscopy
KW  - RNA structures
KW  - site-directed spin labeling
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/63849
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-638497
SN  - 1521-3773
N1  - We acknowledge financial support from the German Research Foundation (CRC902: Molecular Principles of RNA Based Regulation). A.C. thanks Heike Biehl and Anja Becker (MPI of Biophysics) for the training with the microinjection procedure and for having provided the oocytes. S.Th.S. acknowledges financial support by the Icelandic Research Fund (141062051). S.v.B, L.S.S. and G.H. acknowledge support by the Max Planck Society and by the Human Frontier Science Program RGP0026/2017. Open access funding enabled and organized by Projekt DEAL.
VL  - 59
IS  - 51
SP  - 23025
EP  - 23029
PB  - Wiley-VCH
CY  - Weinheim
ER  -