TY  - JOUR
A1  - Herkt, Stefanie
A1  - Lausen, Olga Nikolaevna
A1  - Herglotz, Julia
A1  - Schneider, Lucas
A1  - Meyer, Annekarin
A1  - Pommerenke, Claudia
A1  - Salinas-Riester, Gabriela
A1  - Seifried, Erhard
A1  - Bönig, Halvard-Björn
A1  - Lausen, Jörn
T1  - Protein arginine methyltransferase 6 controls brythroid Ggene expression and differentiation of human CD34+ progenitor cells
T2  - Haematologica
N2  - Hematopoietic differentiation is driven by transcription factors, which orchestrate a finely tuned transcriptional network. At bipotential branching points lineage decisions are made, where key transcription factors initiate cell type-specific gene expression programs. These programs are stabilized by the epigenetic activity of recruited chromatin-modifying cofactors. An example is the association of the transcription factor RUNX1 with protein arginine methyltransferase 6 (PRMT6) at the megakaryocytic/erythroid bifurcation. However, little is known about the specific influence of PRMT6 on this important branching point. Here, we show that PRMT6 inhibits erythroid gene expression during megakaryopoiesis of primary human CD34+ progenitor cells. PRMT6 is recruited to erythroid genes, such as glycophorin A. Consequently, a repressive histone modification pattern with high H3R2me2a and low H3K4me3 is established. Importantly, inhibition of PRMT6 by shRNA or small molecule inhibitors leads to upregulation of erythroid genes and promotes erythropoiesis. Our data reveal that PRMT6 plays a role in the control of erythroid/megakaryocytic differentiation and open up the possibility that manipulation of PRMT6 activity could facilitate enhanced erythropoiesis for therapeutic use.
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/46391
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-463918
SN  - 1592-8721
SN  - 0390-6078
N1  - ©2018 Ferrata Storti Foundation. Material published in Haematologica is covered by copyright. All rights are reserved to the Ferrata Storti Foundation. Use of published material is allowed under the following terms and conditions: https://creativecommons.org/licenses/by-nc/4.0/legalcode. Copies of published material are allowed for personal or internal use. Sharing published material for non-commercial purposes is subject to the following conditions: https://creativecommons.org/licenses/by-nc/4.0/legalcode, sect. 3. Reproducing and sharing published material for commercial purposes is not allowed without permission in writing from the publisher.
VL  - 103
IS  - 1
SP  - 18
EP  - 29
PB  - Ferrata Storti Found
CY  - Pavia
ER  -