TY  - JOUR
A1  - Liu, Hsiao-Chuan
A1  - Gang, Eun Ji
A1  - Kim, Hye Na
A1  - Ruan, Yongsheng
A1  - Ogana, Heather
A1  - Wan, Zesheng
A1  - Bönig, Halvard-Björn
A1  - Shung, Kirk
A1  - Kim, Yong-Mi
T1  - Characterizing the motility of chemotherapeutics-treated acute lymphoblastic leukemia cells by time-lapse imaging
T2  - Cells
N2  - Drug resistance is an obstacle in the therapy of acute lymphoblastic leukemia (ALL). Whether the physical properties such as the motility of the cells contribute to the survival of ALL cells after drug treatment has recently been of increasing interest, as they could potentially allow the metastasis of solid tumor cells and the migration of leukemia cells. We hypothesized that chemotherapeutic treatment may alter these physical cellular properties. To investigate the motility of chemotherapeutics-treated B-cell ALL (B-ALL) cells, patient-derived B-ALL cells were treated with chemotherapy for 7 days and left for 12 h without chemotherapeutic treatment. Two parameters of motility were studied, velocity and migration distance, using a time-lapse imaging system. The study revealed that compared to non-chemotherapeutically treated B-ALL cells, B-ALL cells that survived chemotherapy treatment after 7 days showed reduced motility. We had previously shown that Tysabri and P5G10, antibodies against the adhesion molecules integrins α4 and α6, respectively, may overcome drug resistance mediated through leukemia cell adhesion to bone marrow stromal cells. Therefore, we tested the effect of integrin α4 or α6 blockade on the motility of chemotherapeutics-treated ALL cells. Only integrin α4 blockade decreased the motility and velocity of two chemotherapeutics-treated ALL cell lines. Interestingly, integrin α6 blockade did not affect the velocity of chemoresistant ALL cells. This study explores the physical properties of the movements of chemoresistant B-ALL cells and highlights a potential link to integrins. Further studies to investigate the underlying mechanism are warranted.
KW  - acute lymphoblastic leukemia
KW  - chemotherapeutics-treated
KW  - time-lapse imaging
KW  - cell motility
KW  - integrins
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/55430
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-554305
VL  - 9
IS  - (6), 1470;
PB  - MDPI
CY  - Basel
ER  -