TY  - JOUR
A1  - Wiatr, Marie
A1  - Staubach, Simon
A1  - Figueiredo, Ricardo
A1  - Stump-Guthier, Carolin
A1  - Ishikawa, Hiroshi
A1  - Schwerk, Christian
A1  - Schroten, Horst
A1  - Hanisch, Franz-Georg
A1  - Rudolph, Henriette
A1  - Tenenbaum, Tobias
T1  - Echovirus-30 infection alters host proteins in lipid rafts at the cerebrospinal fluid barrier In vitro
T2  - Microorganisms
N2  - Echovirus-30 (E-30) is a non-polio enterovirus responsible for meningitis outbreaks in children worldwide. To gain access to the central nervous system (CNS), E-30 first has to cross the blood-brain barrier (BBB) or the blood-cerebrospinal fluid barrier (BCSFB). E-30 may use lipid rafts of the host cells to interact with and to invade the BCSFB. To study enteroviral infection of the BCSFB, an established in vitro model based on human immortalized brain choroid plexus papilloma (HIBCPP) cells has been used. Here, we investigated the impact of E-30 infection on the protein content of the lipid rafts at the BCSFB in vitro. Mass spectrometry analysis following E-30 infection versus uninfected conditions revealed differential abundancy in proteins implicated in cellular adhesion, cytoskeleton remodeling, and endocytosis/vesicle budding. Further, we evaluated the blocking of endocytosis via clathrin/dynamin blocking and its consequences for E-30 induced barrier disruption. Interestingly, blocking of endocytosis had no impact on the capacity of E-30 to induce loss of barrier properties in HIBCPP cells. Altogether, these data highlight the impact of E-30 on HIBCPP cells microdomain as an important factor for host cell alteration.
KW  - enterovirus
KW  - Echovirus-30
KW  - lipid raft
KW  - blood-cerebrospinal fluid barrier
KW  - viral infection
KW  - HIBCPP cells
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/57480
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-574806
SN  - 2076-2607
VL  - 8
IS  - Article 1958
PB  - MDPI
CY  - Basel
ER  -