TY  - JOUR
A1  - Blümler, Anja
A1  - Schwalbe, Harald
A1  - Heckel, Alexander
T1  - Solid-phase-supported chemoenzymatic synthesis of a light-activatable tRNA derivative
T2  - Angewandte Chemie. International Edition
N2  - Herein, we present a multi-cycle chemoenzymatic synthesis of modified RNA with simplified solid-phase handling to overcome size limitations of RNA synthesis. It combines the advantages of classical chemical solid-phase synthesis and enzymatic synthesis using magnetic streptavidin beads and biotinylated RNA. Successful introduction of light-controllable RNA nucleotides into the tRNAMet sequence was confirmed by gel electrophoresis and mass spectrometry. The methods tolerate modifications in the RNA phosphodiester backbone and allow introductions of photocaged and photoswitchable nucleotides as well as photocleavable strand breaks and fluorophores.
KW  - chemoenzymatic synthesis
KW  - ligases
KW  - light control
KW  - RNA synthesis
KW  - solid-phase synthesis
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/75119
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-751192
SN  - 1521-3773
N1  - This research was funded by the Deutsche Forschungsgemeinschaft (DFG) through GRK 1986—CliC (Complex Light Control). Work at BMRZ is supported by the state of Hesse. Open Access funding enabled and organized by Projekt DEAL.
VL  - 61
IS  - 1, art. e202111613
SP  - 1
EP  - 6
PB  - Wiley-VCH
CY  - Weinheim
ER  -