TY  - JOUR
A1  - Volz, Yvonne
A1  - Koschut, David
A1  - Matzke-Og, Alexandra
A1  - Dietz, Marina
A1  - Karathanasis, Christos
A1  - Richert, Ludovic
A1  - Wagner, Moritz G.
A1  - Mély, Yves
A1  - Heilemann, Mike
A1  - Niemann, Hartmut H.
A1  - Orian-Rousseau, Véronique
T1  - Direct binding of hepatocyte growth factor and vascular endothelial growth factor to CD44v6
T2  - Bioscience reports
N2  - CD44v6, a member of the CD44 family of transmembrane glycoproteins is a co-receptor for two receptor tyrosine kinases (RTKs), Met and VEGFR-2 (vascular endothelial growth factor receptor 2). CD44v6 is not only required for the activation of these RTKs but also for signalling. In order to understand the role of CD44v6 in Met and VEGFR-2 activation and signalling we tested whether CD44v6 binds to their ligands, HGF (hepatocyte growth factor) and VEGF (vascular endothelial growth factor), respectively. FACS analysis and cellular ELISA showed binding of HGF and VEGF only to cells expressing CD44v6. Direct binding of CD44v6 to HGF and VEGF was demonstrated in pull-down assays and the binding affinities were determined using MicroScale Thermophoresis, fluorescence correlation spectroscopy and fluorescence anisotropy. The binding affinity of CD44v6 to HGF is in the micromolar range in contrast with the high-affinity binding measured in the case of VEGF and CD44v6, which is in the nanomolar range. These data reveal a heparan sulfate-independent direct binding of CD44v6 to the ligands of Met and VEGFR-2 and suggest different roles of CD44v6 for these RTKs.
KW  - binding affinity
KW  - CD44s
KW  - CD44v6
KW  - HGF
KW  - Met
KW  - VEGF
Y1  - 2015
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/44545
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-445453
SN  - 1573-4935
SN  - 0144-8463
N1  - © 2015 Authors This is an open access article published by Portland Press Limited and distributed under the Creative Commons Attribution Licence 3.0
VL  - 35
IS  - 4, e00236
SP  - 1
EP  - 16
PB  - Portland Press
CY  - Colchester
ER  -