TY  - JOUR
A1  - Grünewald, Lena
A1  - Geburtig-Chiocchetti, Andreas
A1  - Weber, Heike
A1  - Scholz, Claus-Jürgen
A1  - Schartner, Christoph
A1  - Freudenberg, Florian
A1  - Reif, Andreas
T1  - Knockdown of the ADHD candidate gene Diras2 in murine hippocampal primary cells
T2  - Journal of attention disorders
N2  - Objective: The DIRAS2 gene is associated with ADHD, but its function is largely unknown. Thus, we aimed to explore the genes and molecular pathways affected by DIRAS2. Method: Using short hairpin RNAs, we downregulated Diras2 in murine hippocampal primary cells. Gene expression was analyzed by microarray and affected pathways were identified. We used quantitative real-time polymerase chain reaction (qPCR) to confirm expression changes and analyzed enrichment of differentially expressed genes in an ADHD GWAS (genome-wide association studies) sample. Results: Diras2 knockdown altered expression of 1,612 genes, which were enriched for biological processes involved in neurodevelopment. Expression changes were confirmed for 33 out of 88 selected genes. These 33 genes showed significant enrichment in ADHD patients in a gene-set-based analysis. Conclusion: Our findings show that Diras2 affects numerous genes and thus molecular pathways that are relevant for neurodevelopmental processes. These findings may further support the hypothesis that DIRAS2 is linked to etiological processes underlying ADHD. (J. of Att. Dis. 2021; 25(4) 572-583).
KW  - expression studies
KW  - neurodevelopmental disorders
KW  - adeno-associated virus
KW  - viral gene transfer
KW  - RNAi
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/57707
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-577071
SN  - 1557-1246
SN  - 1087-0547
VL  - 25
IS  - 4
SP  - 572
EP  - 583
PB  - Sage
CY  - Thousand Oaks, Calif.
ER  -