TY  - JOUR
A1  - Wetzstein, Nils
A1  - Kohl, Thomas A.
A1  - Andres, Sönke
A1  - Schultze, Tilman
A1  - Geil, Ari
A1  - Kim, Eunhee
A1  - Biciusca, Teodora
A1  - Hügel, Christian
A1  - Hogardt, Michael
A1  - Lehn, Annette
A1  - Vehreschild, Maria J. G. T.
A1  - Wolf, Timo
A1  - Niemann, Stefan
A1  - Maurer, Florian Peter
A1  - Wichelhaus, Thomas Alexander
T1  - Comparative analysis of phenotypic and genotypic antibiotic susceptibility patterns in Mycobacterium avium complex
T2  - International journal of infectious diseases
N2  - Objective: Phenotypic (Sensititre Myco, pDST) and genotypic drug susceptibility testing (GenoType NTM DR, gDST) in M. avium complex (MAC) have become available as standardized assays, but comparable data is needed. This study aimed to investigate the phenotypic and genotypic drug susceptibility patterns in MAC clinical isolates.
Methods: Overall, 98 isolates from 85 patients were included. pDST and gDST were performed on all isolates and results compared regarding specificity and sensitivity using pDST as a reference method. The impact of drug instability on pDST results was studied using a biological assay over 14 days. In addition, the evolution of antimicrobial resistance was investigated in sequential isolates of 13 patients.
Results: Macrolide resistance was rare, 1.2% (95% CI 0.7–7.3) of isolates in the base cohort. No aminoglycoside resistances were found, but 14.1% of the studied isolates (95% CI 7.8–23.8) showed intermediate susceptibility. The GenoType NTM DR identified two out of four macrolide-resistant isolates. Antibiotic stability was demonstrated to be poor in rifampicin, rifabutin, and doxycycylin.
Conclusions: pDST results in NTM for unstable antibiotics must be interpreted with care. A combination of pDST and gDST will be useful for the guidance of antimicrobial therapy in MAC-disease.
KW  - NTM
KW  - Non-tuberculous mycobacteria
KW  - M. avium complex
KW  - M. avium
KW  - M. chimaera
KW  - M. Intracellulare
KW  - Drug susceptibility testing
KW  - DST
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/55296
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-552965
SN  - 1201-9712
SN  - 1878-3511
N1  - This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
VL  - 93
SP  - 320
EP  - 328
PB  - Elsevier
CY  - Amsterdam
ER  -