TY  - JOUR
A1  - Lößner, Holger
A1  - Schlattmeier, Insea
A1  - Anders-Maurer, Marie
A1  - Bekeredjian-Ding, Isabelle
A1  - Rohde, Christine
A1  - Wittmann, Johannes
A1  - Pokalyuk, Cornelia
A1  - Krut, Oleg
A1  - Kamp, Christel
T1  - Kinetic fingerprinting links bacteria-phage interactions with emergent dynamics: Rapid depletion of klebsiella pneumoniae indicates phage synergy
T2  - Antibiotics
N2  - The specific temporal evolution of bacterial and phage population sizes, in particular bacterial depletion and the emergence of a resistant bacterial population, can be seen as a kinetic fingerprint that depends on the manifold interactions of the specific phage–host pair during the course of infection. We have elaborated such a kinetic fingerprint for a human urinary tract Klebsiella pneumoniae isolate and its phage vB_KpnP_Lessing by a modeling approach based on data from in vitro co-culture. We found a faster depletion of the initially sensitive bacterial population than expected from simple mass action kinetics. A possible explanation for the rapid decline of the bacterial population is a synergistic interaction of phages which can be a favorable feature for phage therapies. In addition to this interaction characteristic, analysis of the kinetic fingerprint of this bacteria and phage combination revealed several relevant aspects of their population dynamics: A reduction of the bacterial concentration can be achieved only at high multiplicity of infection whereas bacterial extinction is hardly accomplished. Furthermore the binding affinity of the phage to bacteria is identified as one of the most crucial parameters for the reduction of the bacterial population size. Thus, kinetic fingerprinting can be used to infer phage–host interactions and to explore emergent dynamics which facilitates a rational design of phage therapies.
KW  - population dynamics
KW  - mathematical modeling
KW  - Klebsiella pneumoniae
KW  - phage
KW  - emergence
KW  - resistance
KW  - synergistic interaction
KW  - kinetic fingerprint
KW  - phage therapy
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/54288
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-542887
SN  - 2079-6382
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
VL  - 9
IS  - 7, Art. 408
SP  - 1
EP  - 15
PB  - MDPI
CY  - Basel
ER  -