TY  - JOUR
A1  - Alshamleh, Islam
A1  - Krause, Nina
A1  - Richter, Christian
A1  - Kurrle, Nina Susanne
A1  - Serve, Hubert
A1  - Günther, Ulrich L.
A1  - Schwalbe, Harald
T1  - Real‐time NMR spectroscopy for studying metabolism
T2  - Angewandte Chemie. International edition
N2  - Current metabolomics approaches utilize cellular metabolite extracts, are destructive, and require high cell numbers. We introduce here an approach that enables the monitoring of cellular metabolism at lower cell numbers by observing the consumption/production of different metabolites over several kinetic data points of up to 48 hours. Our approach does not influence cellular viability, as we optimized the cellular matrix in comparison to other materials used in a variety of in‐cell NMR spectroscopy experiments. We are able to monitor real‐time metabolism of primary patient cells, which are extremely sensitive to external stress. Measurements are set up in an interleaved manner with short acquisition times (approximately 7 minutes per sample), which allows the monitoring of up to 15 patient samples simultaneously. Further, we implemented our approach for performing tracer‐based assays. Our approach will be important not only in the metabolomics fields, but also in individualized diagnostics.
KW  - biological chemistry
KW  - cell studies
KW  - metabolism
KW  - personalized medicine
KW  - real-time NMR spectroscopy
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/53344
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-533444
SN  - 1521-3773
SN  - 1433-7851
N1  - This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
N1  - Auch als "deutsche Ausgabe" erschienen: Angewandte Chemie, 132.2020, Nr. 6, S. 2324-2328, doi:10.1002/ange.201912919
VL  - 59
IS  - 6
SP  - 2304
EP  - 2308
PB  - Wiley-VCH
CY  - Weinheim
ER  -