TY  - JOUR
A1  - Kornstädt, Lisa
A1  - Pierre, Sandra
A1  - Weigert, Andreas
A1  - Ebersberger, Stefanie
A1  - Schäufele, Tim J.
A1  - Kolbinger, Anja
A1  - Schmid, Tobias
A1  - Cohnen, Jennifer
A1  - Thomas, Dominique Jeanette
A1  - Ferreirós Bouzas, Nerea
A1  - Brüne, Bernhard
A1  - Ebersberger, Ingo
A1  - Scholich, Klaus
T1  - Bacterial and fungal toll-like receptor activation elicits type I IFN responses in mast cells
T2  - Frontiers in immunology
N2  - Next to their role in IgE-mediated allergic diseases and in promoting inflammation, mast cells also have antiinflammatory functions. They release pro- as well as antiinflammatory mediators, depending on the biological setting. Here we aimed to better understand the role of mast cells during the resolution phase of a local inflammation induced with the Toll-like receptor (TLR)-2 agonist zymosan. Multiple sequential immunohistology combined with a statistical neighborhood analysis showed that mast cells are located in a predominantly antiinflammatory microenvironment during resolution of inflammation and that mast cell-deficiency causes decreased efferocytosis in the resolution phase. Accordingly, FACS analysis showed decreased phagocytosis of zymosan and neutrophils by macrophages in mast cell-deficient mice. mRNA sequencing using zymosan-induced bone marrow-derived mast cells (BMMC) revealed a strong type I interferon (IFN) response, which is known to enhance phagocytosis by macrophages. Both, zymosan and lipopolysaccharides (LPS) induced IFN-β synthesis in BMMCs in similar amounts as in bone marrow derived macrophages. IFN-β was expressed by mast cells in paws from naïve mice and during zymosan-induced inflammation. As described for macrophages the release of type I IFNs from mast cells depended on TLR internalization and endosome acidification. In conclusion, mast cells are able to produce several mediators including IFN-β, which are alone or in combination with each other able to regulate the phagocytotic activity of macrophages during resolution of inflammation.
KW  - mast cells
KW  - inflammation
KW  - resolution
KW  - IFN-β
KW  - toll-like receptor
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/60949
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-609493
SN  - 1664-3224
VL  - 11
IS  - art. 607048
SP  - 1
EP  - 15
PB  - Frontiers Media
CY  - Lausanne
ER  -