TY  - JOUR
A1  - Dambek, Michael
A1  - Eilers, Ulrike
A1  - Breitenbach, Jürgen
A1  - Steiger, Sabine
A1  - Büchel, Claudia
A1  - Sandmann, Gerhard
T1  - Biosynthesis of fucoxanthin and diadinoxanthin and function of initial pathway genes in Phaeodactylum tricornutum
T2  - The journal of experimental botany
N2  - The biosynthesis pathway to diadinoxanthin and fucoxanthin was elucidated in Phaeodactylum tricornutum by a combined approach involving metabolite analysis identification of gene function. For the initial steps leading to β-carotene, putative genes were selected from the genomic database and the function of several of them identified by genetic pathway complementation in Escherichia coli. They included genes encoding a phytoene synthase, a phytoene desaturase, a ζ-carotene desaturase, and a lycopene β-cyclase. Intermediates of the pathway beyond β-carotene, present in trace amounts, were separated by TLC and identified as violaxanthin and neoxanthin in the enriched fraction. Neoxanthin is a branching point for the synthesis of both diadinoxanthin and fucoxanthin and the mechanisms for their formation were proposed. A single isomerization of one of the allenic double bounds in neoxanthin yields diadinoxanhin. Two reactions, hydroxylation at C8 in combination with a keto-enol tautomerization and acetylation of the 3′-HO group results in the formation of fucoxanthin.
KW  - carotenogenic genes
KW  - complementation
KW  - diadinoxanthin
KW  - fucoxanthin
KW  - genetic pathway
KW  - neoxanthin
KW  - Phaeodactylum tricornutum
Y1  - 2012
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/25594
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-255945
SN  - 1460-2431
SN  - 0022-0957
VL  - 63
IS  - 15
PB  - Univ. Press
CY  - Oxford
ER  -