TY  - JOUR
A1  - Cinatl, Jindrich
A1  - Michaelis, Martin
A1  - Hernáiz Drievert, Pablo
A1  - Cinatl, Jaroslav
A1  - Hrabeta, Jan
A1  - Suhan, Tatyana
A1  - Doerr, Hans Wilhelm
A1  - Vogel, Jens-Uwe
T1  - Multimutated herpes simplex virus G207 is a potent inhibitor of angiogenesis
T2  - Neoplasia
N2  - The mode of the antitumoral activity of multimutated oncolytic herpes simplex virus type 1 G207 has not been fully elucidated yet. Because the antitumoral activity of many drugs involves the inhibition of tumor blood vessel formation, we determined if G207 had an influence on angiogenesis. Monolayers of human umbilical vein endothelial cells and human dermal microvascular endothelial cells, but not human dermal fibroblasts, bronchial epithelial cells, and retinal glial cells, were highly sensitive to the replicative and cytotoxic effects of G207. Moreover, G207 infection caused the destruction of endothelial cell tubes in vitro. In the in vivo Matrigel plug assay in mice, G207 suppressed the formation of perfused vessels. Intratumoral treatment of established human rhabdomyosarcoma xenografts with G207 led to the destruction of tumor vessels and tumor regression. Ultrastructural investigations revealed the presence of viral particles in both tumor and endothelial cells of G207-treated xenografts, but not in adjacent normal tissues. These findings show that G207 may suppress tumor growth, in part, due to inhibition of angiogenesis.
KW  - Angiogenesis
KW  - HSV-1
KW  - G207
KW  - human rhabdomyosarcoma
KW  - ribonucleotide reductase
Y1  - 2004
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/75952
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-759520
SN  - 1476-5586
VL  - 6
IS  - 6
SP  - 725
EP  - 735
PB  - Elsevier
CY  - Amsterdam
ER  -