TY  - JOUR
A1  - Ridder, Kirsten
A1  - Keller, Sascha
A1  - Dams, Maria
A1  - Rupp, Anne-Kathleen
A1  - Schlaudraff, Jessica
A1  - Del Turco, Domenico
A1  - Starmann, Julia
A1  - Macas, Jadranka
A1  - Karpova, Darja
A1  - Devraj, Kavi
A1  - Depboylu, Candan
A1  - Landfried, Britta
A1  - Arnold, Bernd
A1  - Plate, Karl
A1  - Höglinger, Günter
A1  - Sültmann, Holger
A1  - Altevogt, Peter
A1  - Momma, Stefan
T1  - Extracellular vesicle-mediated transfer of genetic information between the hematopoietic system and the brain in response to inflammation
T2  - PLoS biology
N2  - Mechanisms behind how the immune system signals to the brain in response to systemic inflammation are not fully understood. Transgenic mice expressing Cre recombinase specifically in the hematopoietic lineage in a Cre reporter background display recombination and marker gene expression in Purkinje neurons. Here we show that reportergene expression in neurons is caused by intercellular transfer of functional Cre recombinase messenger RNA from immune cells into neurons in the absence of cell fusion. In vitro purified secreted extracellular vesicles (EVs) from blood cells contain Cre mRNA, which induces recombination in neurons when injected into the brain. Although Cre-mediated recombination events in the brain occur very rarely in healthy animals, their number increases considerably in different injury models, particularly under inflammatory conditions, and extend beyond Purkinje neurons to other neuronal populations in cortex, hippocampus, and substantia nigra. Recombined Purkinje neurons differ in their miRNA profile from their nonrecombined counterparts, indicating physiological significance. These observations reveal the existence of a previously unrecognized mechanism to communicate RNA-based signals between the hematopoietic system and various organs, including the brain, in response to inflammation.
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/33272
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-332723
SN  - 1545-7885
SN  - 1544-9173
N1  - Copyright: © 2014 Ridder et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 12
IS  - (6): e1001874
PB  - Public Library of Science
CY  - Lawrence, KS
ER  -