TY  - JOUR
A1  - Mateescu, Bogdan
A1  - Kowal, Emma
A1  - Balkom, Bastiaan Wilhelmus Maria van
A1  - Bartel, Sabine
A1  - Bhattacharyya, Suvendra N.
A1  - Buzás, Edit I.
A1  - Buck, Amy H.
A1  - de-Candia, Paola
A1  - Chow, Franklin W. N.
A1  - Das, Saumya
A1  - Driedonks, Tom A. P.
A1  - Fernández-Messina, Lola
A1  - Haderk, Franziska
A1  - Hill, Andrew F.
A1  - Jones, Jennifer C.
A1  - Van Keuren-Jensen, Kendall R.
A1  - Lai, Charles P.
A1  - Lässer, Cecilia
A1  - Di Liegro, Italia
A1  - Lunavat, Taral Rameshchand
A1  - Lorenowicz, Magdalena J.
A1  - Maas, Sybren L. N.
A1  - Mäger, Imre
A1  - Mittelbrunn, Maria
A1  - Momma, Stefan
A1  - Mukherjee, Kamalika
A1  - Mukherjee, Kamalika
A1  - Nawaz, Muhammed
A1  - Pegtel, Dirk Michiel
A1  - Pfaff, Michael W.
A1  - Schiffelers, Raymond M.
A1  - Tahara, Hidetoshi
A1  - Théry, Clotilde
A1  - Tosar, Juan Pablo
A1  - Wauben, Marca H. M.
A1  - Witwer, Kenneth W.
A1  - Nolte-’t Hoen, Esther N. M.
T1  - Obstacles and opportunities in the functional analysis of extracellular vesicle RNA - an ISEV position paper
T2  - Journal of extracellular vesicle
N2  - The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has been demonstrated in a multitude of different in vitro cell systems and in a variety of body fluids. RNA-containing EV are in the limelight for their capacity to communicate genetically encoded messages to other cells, their suitability as candidate biomarkers for diseases, and their use as therapeutic agents. Although EV-RNA has attracted enormous interest from basic researchers, clinicians, and industry, we currently have limited knowledge on which mechanisms drive and regulate RNA incorporation into EV and on how RNA-encoded messages affect signalling processes in EV-targeted cells. Moreover, EV-RNA research faces various technical challenges, such as standardisation of EV isolation methods, optimisation of methodologies to isolate and characterise minute quantities of RNA found in EV, and development of approaches to demonstrate functional transfer of EV-RNA in vivo. These topics were discussed at the 2015 EV-RNA workshop of the International Society for Extracellular Vesicles. This position paper was written by the participants of the workshop not only to give an overview of the current state of knowledge in the field, but also to clarify that our incomplete knowledge – of the nature of EV(-RNA)s and of how to effectively and reliably study them – currently prohibits the implementation of gold standards in EV-RNA research. In addition, this paper creates awareness of possibilities and limitations of currently used strategies to investigate EV-RNA and calls for caution in interpretation of the obtained data.
KW  - Extracellular vesicles
KW  - exosomes
KW  - non-coding RNA
KW  - mRNA
KW  - function, sorting
KW  - RNA binding proteins
KW  - quantification
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/43175
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-431759
SN  - 2001-3078
N1  - © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 6
IS  - 1, Art. 1286095
SP  - 1
EP  - 33
PB  - Co-Action Publ.
CY  - [s. l.]
ER  -