TY  - INPR
A1  - Rahm, Johanna Viola
A1  - Balakrishnan, Ashwin
A1  - Wehrheim, Maren
A1  - Kaminer, Alexandra
A1  - Glogger, Marius
A1  - Kessler, Laurell F.
A1  - Kaschube, Matthias
A1  - Barth, Hans-Dieter
A1  - Heilemann, Mike
T1  - Fast and long-term super-resolution imaging of ER nano-structural dynamics in living cells using a neural network
T2  - bioRxiv
N2  - Stimulated emission depletion (STED) microscopy is a super-resolution technique that surpasses the diffraction limit and has contributed to the study of dynamic processes in living cells. However, high laser intensities induce fluorophore photobleaching and sample phototoxicity, limiting the number of fluorescence images obtainable from a living cell. Here, we address these challenges by using ultra-low irradiation intensities and a neural network for image restoration, enabling extensive imaging of single living cells. The endoplasmic reticulum (ER) was chosen as the target structure due to its dynamic nature over short and long timescales. The reduced irradiation intensity combined with denoising permitted continuous ER dynamics observation in living cells for up to 7 hours with a temporal resolution of seconds. This allowed for quantitative analysis of ER structural features over short (seconds) and long (hours) timescales within the same cell, and enabled fast 3D live-cell STED microscopy. Overall, the combination of ultra-low irradiation with image restoration enables comprehensive analysis of organelle dynamics over extended periods in living cells.
Y1  - 2024
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/86491
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-864914
UR  - https://www.biorxiv.org/content/10.1101/2024.07.30.605742v1
IS  - 2024.07.30.605742v1
ER  -