TY  - JOUR
A1  - Botelho, Salomé Calado
A1  - Österberg, Marie
A1  - Reichert, Andreas
A1  - Yamano, Koji
A1  - Björkholm, Patrik
A1  - Endo, Toshiya
A1  - Heijne, Gunnar von
A1  - Kim, Hyun
T1  - TIM23-mediated insertion of transmembrane alpha-helices into the mitochondrial inner membrane
T2  - The EMBO Journal
N2  - While overall hydrophobicity is generally recognized as the main characteristic of transmembrane (TM) alpha-helices, the only membrane system for which there are detailed quantitative data on how different amino acids contribute to the overall efficiency of membrane insertion is the endoplasmic reticulum (ER) of eukaryotic cells. Here, we provide comparable data for TIM23-mediated membrane protein insertion into the inner mitochondrial membrane of yeast cells. We find that hydrophobicity and the location of polar and aromatic residues are strong determinants of membrane insertion. These results parallel what has been found previously for the ER. However, we see striking differences between the effects elicited by charged residues flanking the TM segments when comparing the mitochondrial inner membrane and the ER, pointing to an unanticipated difference between the two insertion systems. Keywords: CoxVa , membrane protein , Mgm1p , mitochondria , TIM23
KW  - CoxVa
KW  - membrane protein
KW  - Mgm1p
KW  - mitochondria
KW  - TIM23
Y1  - 2011
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/16631
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-97828
N1  - This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial No Derivative Works 3.0 Unported License, which permits distribution and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission.
VL  - 30
IS  - 1011
EP  - 1003
ER  -