TY  - INPR
A1  - Spahn, Christoph Klaus
A1  - Grimm, Jonathan B.
A1  - Lavis, Luke D.
A1  - Lampe, Marko
A1  - Heilemann, Mike
T1  - Bleaching-independent, whole-cell, 3D and multi-color STED imaging with exchangeable fluorophores
T2  - bioRxiv
N2  - We demonstrate bleaching-independent STED microscopy using fluorogenic labels that reversibly bind to their target structure. A constant exchange of labels guarantees the removal of photobleached fluorophores and their replacement by intact fluorophores, thereby circumventing bleaching-related limitations of STED super-resolution imaging in fixed and living cells. Foremost, we achieve a constant labeling density and demonstrate a fluorescence signal for long and theoretically unlimited acquisition times. Using this concept, we demonstrate whole-cell, 3D, multi-color and live cell STED microscopy with up to 100 min acquisition time.
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/72500
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-725009
IS  - 420638
ER  -