TY  - JOUR
A1  - Zöller, Nadja Nicole
A1  - Schreiner, Sarah
A1  - Petry, Laura
A1  - Hoffmann, Stephanie
A1  - Steinhorst, Katja
A1  - Kleemann, Johannes
A1  - Jäger, Manuel Alexander
A1  - Kaufmann, Roland
A1  - Meissner, Markus
A1  - Kippenberger, Stefan
T1  - Collagen I promotes adipocytogenesis in adipose-derived stem cells in vitro
T2  - Cells
N2  - hallmark of ageing is the redistribution of body fat. Particularly, subcutaneous fat decreases paralleled by a decrease of skin collagen I are typical for age-related skin atrophy. In this paper, we hypothesize that collagen I may be a relevant molecule stimulating the differentiation of adipose-derived stem cells (ASCs) into adipocytes augmenting subcutaneous fat. In this context lipogenesis, adiponectin, and collagen I receptor expression were determined. Freshly isolated ASCs were characterized by stemness-associated surface markers by FACS analysis and then transdifferentiated into adipocytes by specific medium supplements. Lipogenesis was evaluated using Nile Red staining and documented by fluorescence microscopy or quantitatively measured by using a multiwell spectrofluorometer. Expression of adiponectin was measured by real-time RT-PCR and in cell-free supernatants by ELISA, and expression of collagen I receptors was observed by western blot analysis. It was found that supports coated with collagen I promote cell adhesion and lipogenesis of ASCs. Interestingly, a reverse correlation to adiponectin expression was observed. Moreover, we found upregulation of the collagen receptor, discoidin domain-containing receptor 2; receptors of the integrin family were absent or downregulated. These findings indicate that collagen I is able to modulate lipogenesis and adiponectin expression and therefore may contribute to metabolic dysfunctions associated with ageing.
KW  - adipose-derived stem cells
KW  - adipocytes
KW  - differentiation
KW  - collagen I
KW  - adiponectin
KW  - integrins
KW  - discoidin domain-containing receptor
KW  - ageing
KW  - subcutaneous fat
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/51013
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-510136
SN  - 2073-4409
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
VL  - 8
IS  - 4, Art. 302
SP  - 1
EP  - 13
PB  - MDPI
CY  - Basel
ER  -