TY  - JOUR
A1  - Leucht, Philipp
A1  - Kim, Jae-Beom
A1  - Currey, Jennifer A.
A1  - Brunski, John
A1  - Helms, Jill A.
T1  - FAK-mediated mechanotransduction in skeletal regeneration
T2  - PLoS One
N2  - The majority of cells are equipped to detect and decipher physical stimuli, and then react to these stimuli in a cell type-specific manner. Ultimately, these cellular behaviors are synchronized to produce a tissue response, but how this is achieved remains enigmatic. Here, we investigated the genetic basis for mechanotransduction using the bone marrow as a model system. We found that physical stimuli produced a pattern of principal strain that precisely corresponded to the site-specific expression of sox9 and runx2, two transcription factors required for the commitment of stem cells to a skeletogenic lineage, and the arrangement and orientation of newly deposited type I collagen fibrils. To gain insights into the genetic basis for skeletal mechanotransduction we conditionally inactivated focal adhesion kinase (FAK), an intracellular component of the integrin signaling pathway. By doing so we abolished the mechanically induced osteogenic response and thus identified a critical genetic component of the molecular machinery required for mechanotransduction. Our data provide a new framework in which to consider how physical forces and molecular signals are synchronized during the program of skeletal regeneration.
Y1  - 2007
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/22665
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-115105
SN  - 1932-6203
N1  - Copyright: © 2007 Leucht et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 2
IS  - (4): e390
SP  - 1
EP  - 7
PB  - PLoS
CY  - Lawrence, Kan.
ER  -