TY  - JOUR
A1  - Dücker, Ruth Pia
A1  - Adam, Elisabeth
A1  - Wirtz, Sarah
A1  - Gronau, Lucia
A1  - Khodamoradi, Yascha
A1  - Eberhardt, Fabian J.
A1  - Donath, Helena
A1  - Gutmann, Desiree
A1  - Vehreschild, Maria J. G. T.
A1  - Zacharowski, Kai
A1  - Kreyenberg, Hermann
A1  - Geburtig-Chiocchetti, Andreas
A1  - Zielen, Stefan
A1  - Schubert, Ralf
T1  - The MiR-320 family is strongly downregulated in patients with COVID-19 induced severe respiratory failure
T2  - International journal of molecular sciences
N2  - A high incidence of thromboembolic events associated with high mortality has been reported in severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) infections with respiratory failure. The present study characterized post-transcriptional gene regulation by global microRNA (miRNA) expression in relation to activated coagulation and inflammation in 21 critically ill SARS-CoV-2 patients. The cohort consisted of patients with moderate respiratory failure (n = 11) and severe respiratory failure (n = 10) at an acute stage (day 0–3) and in the later course of the disease (>7 days). All patients needed supplemental oxygen and severe patients were defined by the requirement of positive pressure ventilation (intubation). Levels of D-dimers, activated partial thromboplastin time (aPTT), C-reactive protein (CRP), and interleukin (IL)-6 were significantly higher in patients with severe compared with moderate respiratory failure. Concurrently, next generation sequencing (NGS) analysis demonstrated increased dysregulation of miRNA expression with progression of disease severity connected to extreme downregulation of miR-320a, miR-320b and miR-320c. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis revealed involvement in the Hippo signaling pathway, the transforming growth factor (TGF)-β signaling pathway and in the regulation of adherens junctions. The expression of all miR-320 family members was significantly correlated with CRP, IL-6, and D-dimer levels. In conclusion, our analysis underlines the importance of thromboembolic processes in patients with respiratory failure and emphasizes miRNA-320s as potential biomarkers for severe progressive SARS-CoV-2 infection.
KW  - miRNA
KW  - SARS-CoV-2
KW  - lung disease
KW  - respiratory failure
KW  - D-dimer
KW  - CRP
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/63396
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-633965
SN  - 1422-0067
N1  - This research was funded by Starke Lunge Foundation. Starke Lunge is a non-profitable foundation registered in Germany (Regierungspräsidium Darmstadt: AZ: I 1325d 04/11(6)-78) that supports research projects in pediatric rare lung diseases.
VL  - 22
IS  - 19, art. 10351
SP  - 1
EP  - 14
PB  - Molecular Diversity Preservation International
CY  - Basel
ER  -