TY  - JOUR
A1  - Schiwy, Sabrina
A1  - Herber, Ann-Kathrin
A1  - Hollert, Henner
A1  - Brinkmann, Markus
T1  - New insights into the toxicokinetics of 3,4-dichloroaniline in early life stages of zebrafish (Danio rerio)
T2  - Toxics
N2  - In the fish embryo toxicity (FET) test with zebrafish (Danio rerio) embryos, 3,4-dichloroaniline (3,4-DCA) is often employed as a positive control substance. Previous studies have characterized bioconcentration and transformation of 3,4-DCA in this test under flow-through conditions. However, the dynamic changes of chemical concentrations in exposure media and embryos were not studied systematically under the commonly used semi-static exposure conditions in multiwell plates. To overcome these limitations, we conducted semi-static exposures experiments where embryolarval zebrafish were exposed to 0.5, 2.0, and 4.0 mg L−1 of 3,4-DCA for up to 120 hpf, with 24-h renewal intervals. During each renewal interval, concentrations of 3,4-DCA were quantified in water samples at 0, 6, 18, and 24 h using high-performance liquid chromatography with diode array detection. Levels of 3,4-DCA in larvae were measured after 120 h exposure. Concentrations of 3,4-DCA in the test vessels decreased rapidly during exposure. Taking these dynamics into account, bioconcentration factors in the present study ranged from 12.9 to 29.8 L kg−1, depending on exposure concentration. In summary, this study contributed to our knowledge of chemical dynamics in the FET test with embryolarval zebrafish, which will aid in defining suitable exposure conditions for future studies.
KW  - 3,4-DCA; biotransformation
KW  - FET
KW  - elimination rate constant
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/55582
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-555828
SN  - 2305-6304
VL  - 8
IS  - (1), article 16
PB  - MDPI
CY  - Basel
ER  -