TY  - JOUR
A1  - Yadav, Shivam
A1  - Centola, Martin
A1  - Glaesmann, Mathilda Elisabeth
A1  - Pogoryelov, Denys
A1  - Ladig, Roman
A1  - Heilemann, Mike
A1  - Rai, L. C.
A1  - Yildiz, Özkan
A1  - Schleiff, Enrico
T1  - Cyclophilin anaCyp40 regulates photosystem assembly and phycobilisome association in a cyanobacterium
T2  - Nature Communications
N2  - Cyclophilins, or immunophilins, are proteins found in many organisms including bacteria, plants and humans. Most of them display peptidyl-prolyl cis-trans isomerase activity, and play roles as chaperones or in signal transduction. Here, we show that cyclophilin anaCyp40 from the cyanobacterium Anabaena sp. PCC 7120 is enzymatically active, and seems to be involved in general stress responses and in assembly of photosynthetic complexes. The protein is associated with the thylakoid membrane and interacts with phycobilisome and photosystem components. Knockdown of anacyp40 leads to growth defects under high-salt and high-light conditions, and reduced energy transfer from phycobilisomes to photosystems. Elucidation of the anaCyp40 crystal structure at 1.2-Å resolution reveals an N-terminal helical domain with similarity to PsbQ components of plant photosystem II, and a C-terminal cyclophilin domain with a substrate-binding site. The anaCyp40 structure is distinct from that of other multi-domain cyclophilins (such as Arabidopsis thaliana Cyp38), and presents features that are absent in single-domain cyclophilins.
KW  - Bacterial structural biology
KW  - Cellular microbiology
KW  - Chaperones
KW  - Photosynthesis
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/69536
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-695368
SN  - 2041-1723
N1  - This work was supported by grants from the Deutsche Forschungsgemeinschaft (DFG SCHL585/7-2)
N1  - Open Access funding enabled and organized by Projekt DEAL.
N1  - The atomic coordinates for anaCyp40ΔTM have been deposited in the Protein Data Bank with the accession code 7A73. The source data underlying Figs. 1, 2, 3, 5 are provided as a Source data file. Other data that support the findings of this study are available upon request to the corresponding author. Source data are provided with this paper.
VL  - 13
IS  - art. 1690
SP  - 1
EP  - 17
PB  - Nature Publishing Group UK
CY  - [London]
ER  -