TY  - JOUR
A1  - Wurzel, Patrick
A1  - Ackermann, Jörg
A1  - Schäfer, Hendrik
A1  - Scharf, Sonja
A1  - Hansmann, Martin-Leo
A1  - Koch, Ina
T1  - Detection of follicular regions in actin-stained whole slide images of the human lymph node by shock filter
T2  - Biological chemistry
N2  - Human lymph nodes play a central part of immune defense against infection agents and tumor cells. Lymphoid follicles are compartments of the lymph node which are spherical, mainly filled with B cells. B cells are cellular components of the adaptive immune systems. In the course of a specific immune response, lymphoid follicles pass different morphological differentiation stages. The morphology and the spatial distribution of lymphoid follicles can be sometimes associated to a particular causative agent and development stage of a disease. We report our new approach for the automatic detection of follicular regions in histological whole slide images of tissue sections immuno-stained with actin. The method is divided in two phases: (1) shock filter-based detection of transition points and (2) segmentation of follicular regions. Follicular regions in 10 whole slide images were manually annotated by visual inspection, and sample surveys were conducted by an expert pathologist. The results of our method were validated by comparing with the manual annotation. On average, we could achieve a Zijbendos similarity index of 0.71, with a standard deviation of 0.07.
KW  - computer vision
KW  - digital pathology
KW  - human lymph node
KW  - morphological filtering
KW  - shock filter
KW  - whole slide image
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62828
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-628281
SN  - 1437-4315
N1  - The implementation is available under the following link: https://sourceforge.net/projects/fldetect/.
N1  - This investigation was supported by the Wilhelm Sander Stiftung No. 2018.101.1, BMBF, COMPLS2-087, Patho234.
VL  - 402
IS  - 8
SP  - 991
EP  - 999
PB  - de Gruyter
CY  - Berlin [u.a.]
ER  -