TY  - JOUR
A1  - Thomos, Miguel
A1  - Wurzel, Patrick
A1  - Scharf, Sonja
A1  - Koch, Ina
A1  - Hansmann, Martin-Leo
T1  - 3D investigation shows walls and wall-like structures around human germinal centres, probably regulating T- and B-cell entry and exit
T2  - PLoS ONE
N2  - This study deals with 3D laser investigation on the border between the human lymph node T-zone and germinal centre. Only a few T-cells specific for antigen selected B-cells are allowed to enter germinal centres. This selection process is guided by sinus structures, chemokine gradients and inherent motility of the lymphoid cells. We measured gaps and wall-like structures manually, using IMARIS, a 3D image software for analysis and interpretation of microscopy datasets. In this paper, we describe alpha-actin positive and semipermeable walls and wall-like structures that may hinder T-cells and other cell types from entering germinal centres. Some clearly defined holes or gaps probably regulate lymphoid traffic between T- and B-cell areas. In lymphadenitis, the morphology of this border structure is clearly defined. However, in case of malignant lymphoma, the wall-like structure is disrupted. This has been demonstrated exemplarily in case of angioimmunoblastic T-cell lymphoma. We revealed significant differences of lengths of the wall-like structures in angioimmunoblastic T-cell lymphoma in comparison with wall-like structures in reactive tissue slices. The alterations of morphological structures lead to abnormal and less controlled T- and B-cell distributions probably preventing the immune defence against tumour cells and infectious agents by dysregulating immune homeostasis.
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62395
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-623959
SN  - 1932-6203
VL  - 15
IS  - 11, art. e0242177
SP  - 1
EP  - 11
PB  - PLOS
CY  - San Francisco, California, US
ER  -