TY  - JOUR
A1  - Leiblein, Maximilian
A1  - Koch, Elias Andreas Thomas
A1  - Winkenbach, Andreas
A1  - Schaible, Alexander
A1  - Nau, Christoph
A1  - Büchner, Hubert
A1  - Schröder, Katrin
A1  - Marzi, Ingo
A1  - Henrich, Dirk
T1  - Size matters: Effect of granule size of the bone graft substitute (Herafill®) on bone healing using Masquelet's induced membrane in a critical size defect model in the rat's femur
T2  - Journal of biomedical materials research
N2  - The Masquelet technique for the treatment of large bone defects is a two‐stage procedure based on an induced membrane. The size of a scaffold is reported to be a critical factor for bone healing response. We therefore aimed to investigate the influence of the granule size of a bone graft substitute on bone marrow derived mononuclear cells (BMC) supported bone healing in combination with the induced membrane. We compared three different sizes of Herafill® granules (Heraeus Medical GmbH, Wehrheim) with or without BMC in vivo in a rat femoral critical size defect. A 10 mm defect was made in 126 rats and a membrane induced by a PMMA‐spacer. After 3 weeks, the spacer was taken out and membrane filled with different granule sizes. After 8 weeks femurs were taken for radiological, biomechanical, histological, and immunohistochemical analysis. Further, whole blood of the rat was incubated with granules and expression of 29 peptide mediators was assessed. Smallest granules showed significantly improved bone healing compared to larger granules, which however did not lead to an increased biomechanical stability in the defect zone. Small granules lead to an increased accumulation of macrophages in situ which could be assigned to the inflammatory subtype M1 by majority. Increased release of chemotactic respectively proangiogenic active factors in vitro compared to syngenic bone and beta‐TCP was observed. Granule size of the bone graft substitute Herafill® has significant impact on bone healing of a critical size defect in combination with Masquelet's technique in terms of bone formation and inflammatory.
KW  - bone marrow derived mononuclear cells
KW  - critical size defect
KW  - induced membrane
KW  - Masquelet technique
KW  - scaffold size
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/57205
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-572059
SN  - 1552-4981
VL  - 108.2020
IS  - 10
SP  - 1469
EP  - 1482
PB  - Wiley
CY  - Hoboken, NJ
ER  -